Robert A. Bonomo, MD

  • Professor of Medicine, Pharmacology, Molecular Biology and Microbiology, Case Western Reserve University
  • Chief, Medical Service, Louis Stokes Cleveland Department of Veteran Affairs Medical Center
  • Vice Chair for Veteran Affairs, Department of Medicine, University Hospitals Cleveland Medical Center

Biography

Bacterial resistance to beta-lactams is threatening the most potent antibiotics we have. The research interests in our laboratory involve understanding the structure function relationships of the class A beta-lactamase, SHV-1. This chromosomal and plasmid encoded beta-lactamase is usually found in Klebsiella pneumonia and confers high level resistance to third generation cephalosporins (cefotaxime, ceftazidime, and aztreonam). This resistance can render ineffective the most frequently used drugs to treat serious nosocomial infections. Our goals are to understand what amino acid substitutions permit evolution of novel substrate profiles and what factors control expression of these periplasmic enzymes. By using site directed mutagenesis and immunological tools to quantify expression, we are able to draw conclusions as to why these highly resistant variants may have arisen in nature. We are also interested in quantifying beta-lactamase expression in organisms harboring Class C beta-lactamases. We have developed antibodies able to detect the presence of this class of enzymes in clinical strains, thereby facilitating molecular epidemiological investigations.

Education

B.A.
Hamilton College
M.D.
Case Western Reserve University
Residency
Internal Medicine, University Hospitals of Cleveland
Fellowship
Infectious Diseases, University Hospitals of Cleveland